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. 2014 Sep 8;9(9):e106724. doi: 10.1371/journal.pone.0106724

Figure 2. Heterologous expression of His-MBP-GIFT15 in Rosetta-gami (DE3)pLysS.

Figure 2

(A) SDS-PAGE and (B) Western blot analysis. Soluble fractions after (1) 1 h of induction with IPTG; (2) after 3 h of induction; (3) after 16 h of induction. Immunodetection was conducted with anti-mouse IL-15 antibody.