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. 2014 Sep 8;9(9):e106724. doi: 10.1371/journal.pone.0106724

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© 2014 Pennati et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Purification of His-MBP-GIFT15 on Nickel-Sepharose (A) and on DEAE-Sepharose (B) after cleavage with TEV protease. Black traces are the absorbance readings recorded at 280 nm and red traces represent the elution gradient. (C) SDS-PAGE following His-MBP-GIFT15 purification. (1) cell-free extract before induction with IPTG, (2) cell-free extract after induction with IPTG, (3) His-MBP-GIFT15 after Ni-Sepharose, (4) rGIFT15 after TEV protease treatment and isolation on DEAE-Sepharose, (5) molecular weight markers.