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. 2014 Sep 8;9(9):e106693. doi: 10.1371/journal.pone.0106693

Figure 5. Insulin measurements on individual islets confirms in vivo BLI and insulin measurements.

Figure 5

A) Bioluminescence imaging of islets isolated from ob/ob luciferase mice at 10, 22, 40, and 64 weeks of age displayed increased bioluminescence with increasing age up to 40 weeks of age, followed by decreased BLI at 64 weeks of age. B) Isolated islets displayed increased total insulin content with increasing age up to 40 weeks of age. Insulin content of islets from 64 week old mice was not significantly different than at 40 weeks. C) Isolated ob/ob-luc islets displayed insulin release in response to insulin secretagogues. The response from 64 week old islets is represented by open symbols to distinguish it from the other groups. High glucose (16.7 mM) and IBMX induced higher insulin release in islets from 40 week old mice and lower insulin release in islets from 64 week old mice (*p = 0.015, One way ANOVA).