Table 1. Coefficient of Variation for Area Under the Curve measurements of i.p. vs. i.v. administration of D-luciferin in ob/ob -luc mice.

	CV for Entire AUC (%)	CV for AUC from 5–10 min (%)
IP	51.2±22.3	57.2±26.7
IV	8.3±6.7	10.29±6.5

Test-retest scans were performed every other day for three days on a total of 12 animals. I.P. administration was tested the first week and i.v. administration was tested the following week and dynamic profiles were generated as described in Figure S2. AUC for the entire time course and for just 5–10 min interval was measured for each day and the Coefficient of Variation (CV)  =  ((STD/AUC)*100) was calculated for all three days for each mouse. The CV was then averaged for all 12 mice. I.V. is more consistent than i.p. and there is no statistical significant difference in performing a 5 min acquisition 5 min after D-luciferin administration vs. measuring for the entire time course.