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. 2014 Sep 8;9(9):e106756. doi: 10.1371/journal.pone.0106756

Figure 1. EV71-specific T cell responses induced by EV71vac vaccination.

Figure 1

PBMCs from monkeys immunized with high (n = 4) or low doses of EV71vac (n = 4) or PBS control (n = 2) were stimulated with inactivated EV71 virus or medium alone. Specific IFN-γ or IL-4 production responding to EV71 antigen stimulation were assayed by ELISPOT, and the number of spot forming cells per million subtracted from the medium alone is represented (A and B). At week 6, PBMCs from some of the high or low dose EV71vac-immunized monkeys (n = 3) or PBS controls (n = 1) were assayed for the percentage of TNF-producing CD4+ T cells by intracellular staining and gated on CD3+CD4+ population. The typical result for TNF-producing CD4+ T cells (CD95+ TNF+) from one high dose (A31) and one low dose (A11) subject after stimulation with medium, inactivated EV71 or PMA plus ionomycin is shown (C). Data for EV71-specific TNF-producing CD4+ T cells from all monkeys are summarized in (D).