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. Author manuscript; available in PMC: 2014 Sep 8.
Published in final edited form as: Oncogene. 2012 Jan 16;31(43):4619–4629. doi: 10.1038/onc.2011.612

Figure 4.

Figure 4

SIRT1 silencing by siRNA reduces EMT-inducing transcription factor expression. The mRNA levels of EMT-inducing transcription factors including ZEB1, ZEB2, Snail and Slug were measured by quantitative RT–PCR analysis of 5 μg RNA extracted from DU145 and PC3 cells with stable knockdown of SIRT1 (siSIRT1) or RNAi vector control-infected cells (siVector). The mRNA levels of ZEB1, ZEB2, Snail and Slug are expressed relative to β-actin transcripts. Each experiment was performed in triplicate and repeated three times. The error bars represent the s.e.m. Asterisks indicate significant differences between two groups (*P < 0.05).