Figure 2. 2-D analysis reveals differential proteomic response of A. fumigatus to a combination of gliotoxin and H₂O₂.

2-D proteome maps of A. fumigatus ATCC26933 (a) solvent control, (b) following exposure to gliotoxin (10 µg/ml) for 4 h, (c) following exposure to 2 mM H₂O₂ for 4 h, (d) following exposure to a combination of gliotoxin (10 µg/ml) and H₂O₂ (2 mM) for 4 h. The proteins were first separated on pH 4–7 strips followed by SDS-PAGE. Proteins found to be significantly differentially expressed (p<0.05), after analysis using Progenesis SameSpot software, are numbered. (e) Increased expression of the gliotoxin oxidoreductase GliT in response to gliotoxin but not H₂O₂. GliT expression was increased following exposure to exogenous gliotoxin alone (5.1 fold) and in combination with H₂O₂ (4.8 fold), relative to the solvent control. No significant difference in expression of GliT was detected upon exposure of A. fumigatus to H₂O₂ alone, relative to the control (p>0.05), indicating GliT expression is mediated by gliotoxin only.