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(a) Neither methanol (solvent control) or gliotoxin induce significant ROS formation in A. fumigatus, however H2O2 exposure leads to clear formation of ROS. Co-addition of gliotoxin dissipates ROS as judged by reduced fluorescence. (b) Gliotoxin significantly reduces H2O2-induced ROS levels during co-incubation with H2O2 (p>0.0001).
