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. 2014 Sep 8;9(9):e106942. doi: 10.1371/journal.pone.0106942

Table 2. Proteins (n = 13) exhibiting significant differential abundance1 in A. fumigatus ATCC26933 following the co-addition of gliotoxin and H2O2, relative to H2O2 alone.

Protein Name Co-addition v Control (iii) Co-addition v Gliotoxin (iv) Co-addition v H2O2 (v) H2O2 v Control CADRE ID. (AFUA_) Spot No.
Increased Abundance Proteins in Co-addition v H2O2
Thioredoxin reductase GliT ↑ 4.8 - 3.5 - 6G09740 738
Unknown function protein ↑ 4.4 - 4.9 - 2G11120 803
Proliferating cell nuclear antigen (PCNA) ↑ 5.9 ↑ 7.4 2.4 - 1G04900 980
Ran-specific GTPase-activating protein 1 - - 1.8 - 5G12180 850
NADH-quinone oxidoreductase (23 kDa subunit) - - 1.9 - 1G06610 897
HAD family hydrolase ↑ 1.8 ↑ 2.1 1.5 - 5G08270 989
Decreased Abundance Proteins in Co-addition v H2O2
Unknown function protein - - 2.9 ↑ 2.3 6G03460 964
Molecular chaperone and allergen Mod-E/Hsp90/Hsp1 ↓ 1.5 - 2.7 ↑ 2.0 5G04170 966
Oxidative stress protein Svf1 - - 1.6 - 5G11820 975
Glutamine amidotransferase: cyclase ↓ 1.9 - 1.7 - 2G06230 968
Glycyl-tRNA synthetase ↓ 1.9 - 1.5 - 5G05920 305
Methylenetetrahydrofolate reductase ↓ 1.9 - 1.6 - 2G11300 379
Class V chitinase - - 1.8 - 3G11280 662

Data extracted from Tables S3 and S4 and re-charted for clarity. Proteins detected with a significant change in abundance in H2O2 compared to the control are also reported.

1

p<0.05; Fold increase (↑) or decrease (↓) of protein in the co-additive condition, relative to the solvent control, gliotoxin alone or H2O2 alone. Co-addition: incubation with both gliotoxin and H2O2. CADRE ID., A. fumigatus gene annotation nomenclature according to [1] and [71]; Spot No, according to Figure 2. Numbers in bold indicate fold change of proteins (n = 13) differentially regulated in the co-addition, relative to H2O2 alone.

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