Table 2. Proteins (n = 13) exhibiting significant differential abundance1 in A. fumigatus ATCC26933 following the co-addition of gliotoxin and H2O2, relative to H2O2 alone.
Protein Name | Co-addition v Control (iii) | Co-addition v Gliotoxin (iv) | Co-addition v H2O2 (v) | H2O2 v Control | CADRE ID. (AFUA_) | Spot No. |
Increased Abundance Proteins in Co-addition v H2O2 | ||||||
Thioredoxin reductase GliT | ↑ 4.8 | - | ↑ 3.5 | - | 6G09740 | 738 |
Unknown function protein | ↑ 4.4 | - | ↑ 4.9 | - | 2G11120 | 803 |
Proliferating cell nuclear antigen (PCNA) | ↑ 5.9 | ↑ 7.4 | ↑ 2.4 | - | 1G04900 | 980 |
Ran-specific GTPase-activating protein 1 | - | - | ↑ 1.8 | - | 5G12180 | 850 |
NADH-quinone oxidoreductase (23 kDa subunit) | - | - | ↑ 1.9 | - | 1G06610 | 897 |
HAD family hydrolase | ↑ 1.8 | ↑ 2.1 | ↑ 1.5 | - | 5G08270 | 989 |
Decreased Abundance Proteins in Co-addition v H2O2 | ||||||
Unknown function protein | - | - | ↓ 2.9 | ↑ 2.3 | 6G03460 | 964 |
Molecular chaperone and allergen Mod-E/Hsp90/Hsp1 | ↓ 1.5 | - | ↓ 2.7 | ↑ 2.0 | 5G04170 | 966 |
Oxidative stress protein Svf1 | - | - | ↓ 1.6 | - | 5G11820 | 975 |
Glutamine amidotransferase: cyclase | ↓ 1.9 | - | ↓ 1.7 | - | 2G06230 | 968 |
Glycyl-tRNA synthetase | ↓ 1.9 | - | ↓ 1.5 | - | 5G05920 | 305 |
Methylenetetrahydrofolate reductase | ↓ 1.9 | - | ↓ 1.6 | - | 2G11300 | 379 |
Class V chitinase | - | - | ↓ 1.8 | - | 3G11280 | 662 |
Data extracted from Tables S3 and S4 and re-charted for clarity. Proteins detected with a significant change in abundance in H2O2 compared to the control are also reported.
p<0.05; Fold increase (↑) or decrease (↓) of protein in the co-additive condition, relative to the solvent control, gliotoxin alone or H2O2 alone. Co-addition: incubation with both gliotoxin and H2O2. CADRE ID., A. fumigatus gene annotation nomenclature according to [1] and [71]; Spot No, according to Figure 2. Numbers in bold indicate fold change of proteins (n = 13) differentially regulated in the co-addition, relative to H2O2 alone.