Table 1. Biochemical and conformational properties of ETHE1.
ETHE1-WT | ETHE1-p.Arg163Trp | ETHE1-p.Arg163Gln | |
Specific Activity (U.mg−1) | 21.0±3.0 | 1.8±0.9 | 2.4±0.9 |
Iron content (mol Fe/mol protein) | 1.1±0.1 | 1.2±0.1 | 1.2±0.3 |
Thermal stability Tm (°C) | 62±2 | 52±2 | 55±2 |
Reduction Potential E0 (mV) | −272±15 | −370±15 | −310±15 |
The values presented for the different properties correspond to an average of at least three independent determinations (n = 3).