Figure 3. IL-1 is not required for recruitment of immune cells to the lung.

Lungs were excised and made into a single cell suspension for flow cytometry at the indicated days post-inoculation. Cells were stained with anti-CD45 to identify hematopoietic cells and anti-CD11b, CD3, CD4, CD8, Ly6G, and F4/80 for further subsets. Gating for T cells subsets was performed on CD45⁺CD3⁺ cells with quadrant gating to identify either (A) CD4⁺ or (B) CD8⁺ single-positive T cells. Gating on CD45⁺CD11b⁺ cells, (C) Ly6G⁺ neutrophils or (D) F4/80⁺ macrophages were identified. Data was collected with a BD LSRII flow cytometer. Bars indicate the mean percentage of each group (n = 6) with (*) indicating p<0.05 using the Kruskal-Wallis test with Dunn’s post-hoc test for multiple comparisons.