FIG. 5.

Cell surface and intracellular distribution of capsids in TRVb cell lines stably expressing the wild-type (wt) or mutant feline TfR. The cells were incubated for 1 h at 37°C with CPV capsids and then fixed. Cell-associated virus was detected with Cy2-labeled MAb 8 in Triton X-100-permeabilized or nonpermeabilized cells. The ratio of total virus and surface-accessible virus was determined by flow cytometry. Data are for three independent experiments ±1 standard deviation.