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Nipah virus W is localized to the nucleus and causes redistribution of STAT1 to the nucleus, whereas V and P localize to the cytoplasm and retain STAT1 in the cytoplasm. HeLa cells were transfected with the STAT1 expression plasmid and HA-tagged V, W, or P. Cells were fixed and permeabilized 1 day later. Proteins were stained with antibodies against the HA epitope (left panels) or STAT1 (center panels), and nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (right panels).
