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. 2004 Jun;78(11):5633–5641. doi: 10.1128/JVI.78.11.5633-5641.2004

FIG. 5.

FIG. 5.

FIG. 5.

Nipah virus V, W, and P all inhibit IFN-induced phosphorylation of STAT1. (A) HeLa cells were transfected with a STAT1 plasmid and an HA-tagged V, W, or P plasmid. STAT1 phosphorylation was induced by treatment with 100 ng of IFN-α/ml for 30 min. Proteins were stained with antibodies against the HA epitope (left panels) or pY701-STAT1 (center panels). In cells expressing V or W, there was no evidence of phosphorylated STAT1 in the nucleus (white arrows). Among cells expressing P, some had activated STAT1 in the nucleus (blue arrows), while others did not (yellow arrows). Merged images are shown in the panels on the right. (B) 293T cells were transfected with the indicated plasmids. One day later, STAT1 phosphorylation was induced by treatment with 1,000 IU of IFN-β/ml for 30 min. Cells were lysed, and levels of pY701-STAT1 (upper panel) and bulk STAT1 (lower panel) were determined by Western blot analysis.