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Telomere lengths shorten over the life span of wild-type and mutant E6/E7 cells. Southern blot analysis was performed for telomere restriction fragment (TRF) length on the pBabe vector control, mutant E6/E7, and wild-type E6/E7 keratinocyte lines. Passage number is indicated at the top of each lane. Average TRF length, indicated at the bottom of each lane, was determined using standard calculation methods (1), employing the formula [Σ(length) · (intensity)]/Σ(intensity) for each lane.
