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The triple high affinity oxidase mutant loses viability in biofilms. Flow cell biofilms for wild type and the cioA cbb3-1 cbb3-2 triple mutant PAO1987. SD, side dimension (x,z-plane); TD, top down view (x,y-plane). GFP-labelled biofilms were grown for 96 h at which time dead cells were counterstained with propidium iodide. The combined green (GFP) and red (propidium iodide) channels are presented.
