Table 3.
Effect of oxygen on expression levels of aerobic oxidase and denitrification genes.
| Fold changea | ||||
|---|---|---|---|---|
| Oxygen % | ||||
| Gene no.b | Description | 2 | 0.4 | 0 (+ NO3)c |
| PA0105–PA0108 | coxBA, cytochrome c oxidase, aa3 type and associated proteins | A | A | A |
| PA1317–PA1321 | cyoABCDE, cytochrome o ubiquinol oxidase, cyo type | −8.5 | −9.6 | −17 |
| PA1552–PA1554 | ccoP1Q1O1N1, cytochrome c oxidase, cbb3 type | – | – | −4.2 |
| PA1555–PA1557 | ccoP2Q2O2N2, cytochrome c oxidase, cbb type | 7.2 | 6.2 | – |
| PA3929–PA3930 | cioAB, cyanide insensitive oxidase (CIO) | – | 7.1 | – |
| PA1172–PA1177 | napEFDABC, periplasmic nitrate reductase | – | 7.9 | – |
| PA3872–PA3875 | narK1K2GHJI, respiratory nitrate reductase | – | – | 71 |
| PA0509–PA0519 | nirSMCFDLGHJEN, nitrite reduction | 24 | – | 40 |
| PA0520–PA0522 | nirQOP, nitrite reduction | 11 | – | 86 |
| PA0523–PA0525 | norCBD, nitric-oxide reductase | 40 | – | 255 |
| PA3391–PA3396 | nosRZDFYL, nitrous-oxide reductase | 65 | – | 135 |
a.
Fold changes are expressed in relation to transcript values during growth with 20% oxygen. The fold change shown is the highest change observed for a gene in the operon.
b.
Gene number from the Pseudomonas genome project (http://www.pseudomonas.com).
c.
Cells were grown with 100 mM nitrate.
A, the transcripts for these genes were absent; –, no change in expression.