Table 4.
Genes differentially expressed in response to a decrease in oxygen.
| Fold changeb | ||||
|---|---|---|---|---|
| Oxygen percentage | ||||
| Gene no.a | Descriptiona | 2 | 0.4 | 0 (+ NO3)c |
| PA0050 | Conserved hypothetical protein | 2.6 | 17 | 4.5 |
| PA0459 | Probable ClpA/B protease ATP binding subunit | 7.8 | 23 | 23 |
| PA0529 | Conserved hypothetical protein | – | – | 11 |
| PA0586-0588 | Conserved hypothetical and serine protein kinase | – | 15 | 11 |
| PA0617-0647 | Probable bacteriophage | – | – | 13 |
| PA0713 | Conserved hypothetical protein | 10 | 14 | 7.1 |
| PA0985 | Probable colcin-like toxin | – | – | 25 |
| PA1123 | Conserved hypothetical protein | 18 | 30 | 23 |
| PA1150 | Pyocin S2 | – | – | 13 |
| PA1323-1324 | Conserved hypothetical | – | 11 | 7.7 |
| PA1431 | rsaL | – | 27 | – |
| PA1432 | lasI, autoinducer synthase | – | 30 | – |
| PA1546 | hemN, O2-independent coproporphyrinogen III oxidase | 6.3 | 2.7 | 4.1 |
| PA1673 | Hemerythin | 5.8 | 2.3 | 4.2 |
| PA1746 | Predicted phosphatase | 11 | 20 | 3.4 |
| PA1789 | Universal stress protein, UspA | 5.1 | 2.7 | 3.2 |
| PA1891- 1897 | Conserved hypothetical | – | 25 | – |
| PA2128-2132 | Fimbrial protein synthesis and assembly (CupA) | 12 | 150 | 15 |
| PA2193-2195 | Hydrogen cyanide synthase | 4.0 | 6.0 | – |
| PA2365-2375 | Conserved hypothetical protein and probable Clp protease (PA2371) | 4.4 | 27 | 5.6 |
| PA2381 | Conserved hypothetical protein | 15 | 21 | 33 |
| PA2939 | Probable aminopeptidase | – | 21 | – |
| PA3049 | Ribosome modulation factor | – | 29 | 35 |
| PA3309 | Universal stress protein, UspA | 4.9 | – | – |
| PA3476 | rhlI, autoinducer synthase | – | 5.3 | – |
| PA3569-3560 | mmsBA, 3-OHisobutyrate/methylmalonate semiald DH | 3.5 | 10 | 18 |
| PA3904-3908 | Conserved hypothetical | – | 24 | – |
| PA3911-3913 | Conserved hypothetical protein | 13 | 3.4 | 26 |
| PA3914-3918 | Molybdenum cofactor biosynthesis | – | – | 209 |
| PA4131-4134 | Ferredoxin, CcoN homology, conserved hypotheticals | 8.2 | 19 | – |
| PA4236 | katA, catalase | 4.8 | 4.9 | – |
| PA4306 | Conserved hypothetical protein | – | 20 | – |
| PA4328 | Universal stress protein UspA | 11 | 2.2 | 6.4 |
| PA4352 | Universal stress protein, UspA | 7.4 | – | 4.1 |
| PA4587 | ccpR, cytochrome c551 peroxidase precursor | 15 | 17 | – |
| PA4738-4739 | Conserved hypothetical | – | 25 | 19 |
| PA5027 | Universal stress protein, UspA | 5.7 | 2.0 | 4.6 |
| PA5100 | hutU, urocanase | – | 12 | 3.1 |
| PA5427 | adhA, alcohol dehydrogenase | 9.8 | 7.6 | 12 |
| PA5475 | Conserved hypothetical protein | 9.9 | 6.4 | 10 |
| PA5481-5482 | Conserved hypothetical | – | 22 | 20 |
| PA5496-5497 | Ribonucleotide reductase | 7.8 | 3.1 | 46 |
a.
Genes forming putative operons are grouped and the values shown represent the gene with the highest average expression ratio.
b.
Fold change in gene expression when compared with a culture growing with 20% oxygen, P = 0.001.
c.
The culture was supplemented with 100 mM KNO3.
–, no change in expression.