Table 6.
Bacterial strains and plasmids used for this study.
| Strains or plasmid | Genotype and phenotype | Reference or origin |
|---|---|---|
| P. aeruginosa strains | ||
| PAO1 | Wild-type strain | Jacobs et al. (2003) |
| PTL14859 | ccoN1::ISlacZ/hah, Tcr | Jacobs et al. (2003) |
| PTL12436 | cioA::ISlacZ/hah, Tcr | Jacobs et al. (2003) |
| PTL13321 | anr::ISlacZ/hah, Tcr | Jacobs et al. (2003) |
| PAO1371 | ΔccoNOQP-2; 3088 bp deleted from PA1555–PA1557 | This study |
| PAO1372 | cioA::ISlacZ/hah ΔccoNOQP-2; 3088 bp deleted from PA1555–PA1557, Tcr | This study |
| PAO1373 | ΔccoNOQP-2 ΔccoNOQP-1; 6680 bp deleted from PA1552–PA1557 | This study |
| PAO1387 | cioA::ISlacZ/hah ΔccoNOQP-2 ΔccoNOQP-1; 6680 bp deleted from PA1552–PA1557, Tcr | This study |
| PAO1374 | ΔmucA; mucoid | Starkey and Parsek |
| PAO1-MW1 | rhlI::Tn501 lasI::tetA, Hgr Tc r | Whiteley et al. (1999) |
| PAK | Wild-type strain | Comolli and Donohue (2002) |
| RoxR1 | PAK, roxR ΔSmaI–NsiI::ΩGmr | Comolli and Donohue (2002) |
| E. coli strains | ||
| DH5a | F− λ− recA1 Δ(lacZYA-argF) U169 hsdR17 thi-1 gyrA96 supE44 endA relA1 Φ80dLacZΔM15 | Gibco-BRL |
| S17-1 | thi pro hdsR hdsM+ recA; chromosomal insertion of RP4-2 (Tc::Mu Km::Tn7) | Simon et al. (1983) |
| Plasmids | ||
| pUC19 | High-copy-number cloning vector; Apr | Yanisch-Perron et al. (1985) |
| pEX19Gm | oriT+ sacB+; gene replacement vector with MCS from pUC19; Gmr | Hoang et al. (1998) |
| pPS858 | FRT cassette vector for insertion of selectable markers; Apr, Gmr | Hoang et al. (1998) |
| pFLP2 | sacB+; Flp recombinase-expressing bhr vector; Apr | Hoang et al. (1998) |
| pEX19Ap | oriT+ sacB+; gene replacement vector with MCS from pUC19; Apr | Hoang et al. (1998) |
| pUC-Δcbb3-2 | In-frame deletion of ccoNOPQ-2 cloned into BamHI/XbaI sites of pUC19; Apr |
This study |
| pUC-Δcbb31–2 | In-frame deletion of ccoNOPQ-2 and ccoNOPQ-1 cloned into BamHI/XbaI sites of pUC19; Apr | This study |
| pUC-Δcbb31–2 BamHI | In-frame deletion of ccoNOPQ-2 and ccoNOPQ-1 including an engineered BamHI site cloned into SacI/XbaI sites of pUC19; Apr |
This study |
| pEX-Δcbb3-2 | BamHI/XbaI fragment from pUC-Δcbb3-2 cloned into pEX19Gm; Gmr | This study |
| pEX-Δcbb31–2 | BamHI/XbaI fragment from pUC-Δcbb31–2 cloned into pEX19Gm; Gmr | This study |
| pEX-Δcbb31–2 Gm | SacI/XbaI fragment from pUC Δcbb31–2 BamHI cloned into pEX19Ap; Gmr cassette from pPS858 cloned into the engineered BamHI site of Δcbb31–2; Apr Gmr | This study |