FIG. 5.

Surface-localized p10 is relatively stable. The stabilities of surface-localized p10-2HAN (p10) and the V19M construct were assessed by surface staining of live transfected cells with anti-HA monoclonal antibody. Following antibody addition, the presence of surface-bound antibody specifically attached to the HA-tagged, surface-localized p10 ectodomain was monitored over time (indicated in minutes on the right). Fluorescence images were captured under identical parameters for comparison of the intensities of fluorescence.