Fig. 3. Venn diagrams of miRNAs altered in response to AngII treatment.

(A) AngII activation of the AT₂R in the human kidney cell line affected only four miRNAs, out of which the regulation of three miRNAs was antagonistic in HEK-AT₂R compared to regulation in HEK-AT₁R. (B) AngII activation of AT₂R in RASMCs affected 32 miRNAs. Eighteen of those miRNAs are common to the expression fingerprint observed in the AT₁R RASMCs, albeit the manner of expression is antagonistic. Arrows pointing up (↑) are indicative of an increase in expression while those pointing down (↓) indicate a decrease in expression. A solid line denotes no change. The experimental condition used for obtaining the data in Figure 2A and 2B included 1µM losaratan+1µM AngII, in order to block the endogenous AT₁R expressed in the cell lines and to selectively activate transfected AT₂R in each case.