FIG. 1.

PMO and R9F2-PMO, targeting the MHV polyprotein gene translation initiation codon region, and scrambled control R9F2-PMO were assayed for inhibition of luciferase expression from a chimeric plasmid containing the luciferase gene fused in frame with a 43-base region spanning the MHV polyprotein gene translation initiation codon. The relative percent inhibition by each PMO or R9F2-PMO in one experiment representative of three is shown (A). DBT cells treated with R9F2-PMO-Fl or PMO-Fl were rinsed thoroughly and photographed under mercury vapor illumination to visualize punctate cell-associated fluorescein (B). Fluorescent cells are indicated with black and white arrowheads, respectively, in the phase-contrast and fluorescent image pairs. Fluorescein-positive cells were counted by FACScan. One representative scan from three replicates showing untreated and R9F2-PP PMO-Fl-treated cells is shown (C).