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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 Jul 6;75:58–63. doi: 10.1016/j.yjmcc.2014.06.018

Figure 3. ERK decreases transcription of the SERCA2 promoter.

Figure 3

A. Mouse SERCA2 promoter-luciferase construct show that CREB increases the transcriptional activity of the promoter and ERK1/2 decreases the transcription. All panels in this figure are representative experiments using H9c2 in triplicate, mean + SEM.

B. Mouse SERCA2 promoter-luciferase activity is decreased by cotransfection with a mutant active MEK, and this effect is reduced by MEK inhibitors. PD = PD98059, U = U0126.

C. PE significantly decreases transcription of the SERCA2 promoter, and MEK inhibition prevents the decrease.

D. Mouse SERCA2 promoter-luciferase contransfected with WT PKD, or a mutant active PKD does not have a significant change in transcription.