Table 1. Human primer sequences.
mRNA | Forward Primer | Reverse Primer |
---|---|---|
ANP | 5′- CAG GAT GGA CAG GAT TGG AG -3′ | 5′- TCC TCC CTG GCT GTT ATC TTC -3′ |
BNP | 5′- GCT TTG GGA GGA AGA TGG AC -3′ | 5′- GCA GCC AGG ACT TCC TCT TA -3′ |
CNP | 5′- GGC TCG CCT TCT GCA AGA G -3′ | 5′- GCT TGA GGC CGA AGC AGC CC -3′ |
GC-A | 5′- CGG CAT TCT GAT TGT CTC C -3′ | 5′- ACA GCT CCG AGG CCA GTT -3′ |
GC-B | 5′- CTG CTT TGA TGC CAT AAT TGA C -3′ | 5′- CCA TGT AAG CAT CCC CAA TC -3′ |
NPRC | 5′- ATT GCC ATG ACT GAT GTG GA -3′ | 5′- CGC ATT TCA AAA CGA CCT TC -3′ |
Col I | 5′- GGG ATT CCC TGG ACC TAA AG -3′ | 5′- GGA ACA CCT CGC TCT CCA -3′ |
GUSB | 5′- CCA TTC CTA TGC CAT CGT GT -3′ | 5′- TGG ACC AGG TTG CTG ATG T -3′ |
ANP, atrial natriuretic peptide; BNP, B-type natriuretic peptide; CNP, C-type natriuretic peptide; GC-A, Guanylyl cyclase receptor-A; GC-B, Guanylyl cyclase receptor-B; NPR-C, Natriuretic peptide receptor-C; Col I, Collagen type I; GUSB, Beta-glucuronidase.