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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: Methods. 2014 Apr 18;69(1):76–84. doi: 10.1016/j.ymeth.2014.04.009

Fig. 2.

Fig. 2

An engineered TALEN contains a customizable TALE repeat array that determines its DNA specificity. TALENs typically have a fixed first repeat that targets thymidine (red highlighted), followed by a customized array of TALE repeats (yellow highlighted). Each repeat domain is 32–35 amino acids except the final “0.5” repeat which is only 20 amino acids long. The identity of two amino acids located at positions 12 and 13 in a repeat domain (termed a repeat variable di-residue or RVD) forms a simple code that determines its nucleotide target (as shown in the box).