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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: Methods. 2014 Apr 18;69(1):76–84. doi: 10.1016/j.ymeth.2014.04.009

Fig. 3.

Fig. 3

The construction scheme of the heterodimeric TALEN vectors. Customized TALE repeat arrays generated by REAL, REAL-Fast or FLASH can be cloned into the heterodimeric TALEN expression vectors. For each TALEN pair, researchers should use one (+) and one (−) backbone vector. In addition to a heterodimeric FokI nuclease domain, each expression backbone vector also contains a different 0.5 TALE repeat domain that specifies the last nucleotide of the target sequence (shown in the parentheses).