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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: Methods. 2014 Apr 18;69(1):76–84. doi: 10.1016/j.ymeth.2014.04.009

Fig. 4.

Fig. 4

A representation of T7EI assay results. T7EI assay was performed as described in Section 6.2.1 using single embryo lysis from six nuclease-injected embryos (marked as #1–6) and two uninjected embryos (marked as “U”). An agarose gel image of the results is shown. While the parent amplicons from the uninjected embryos remained intact after the assay, some portions of the parent amplicons from the injected embryos were cleaved by T7EI into two bands of expected sizes. The “fraction cleaved” and the “% modification” calculated according to the protocol are shown at the bottom of each lane. M, 100 bp DNA ladder (New England Biolabs).