FIG. 2.

GFP expression from TGEV recombinant viruses. ST cells were infected with wild-type TGEV, TGEVmut2, or TGEVmut3, incubated for 12 h at 37°C, and subsequently lysed. Harvested lysates were subjected to Western blotting with monoclonal antisera directed against GFP (Clontech) or the TGEV N protein. Murine antisera against the TGEV N protein were raised by immunizing mice with Venezuelan equine encephalitis virus replicon vectors encoding the TGEV N gene.