FIG. 1.

A59 genome organization and replicase antibodies. The A59 genome is 32 kb long. The replicase gene (gene 1, 22 kb) is shown, with replicase protein domains in overlapping ORF1a and ORF1b. Hatched boxes indicate proteinases: PLP1 domain (papain-like proteinase 1), PLP2 domain (papain-like proteinase 2), and 3CLpro (3C-like proteinase). The protein domains for mature proteins p28, p65, p210, p22, Pol, and Hel are indicated. Arrows beneath proteins indicate cleavage by the relevant proteinases. The first cleavage site between p28 and p65 (CS1) is shown. Black rectangles beneath the schematic indicate proteins generated to induce the rabbit polyclonal antibodies used in this study.