FIG. 2.

CJ83193 fails to establish reactivatable latent infection in a mouse ocular model. CD-1 mice were randomly assigned to three groups of six male and six female mice each. Following corneal scarification, individual groups of mice were inoculated with KOS at 10⁶ PFU per eye or with CJ83193 and 7134 at 10⁷ PFU per eye, respectively. (A) Acute viral replication in the eye of CD-1 mice. Both eyes of infected mice were swabbed on days 1, 3, and 5 postinfection. Infectious viruses in eye swab material were assessed by standard plaque assay on Vero cell monolayers for KOS and on U2CEP4R11 cell monolayers for CJ83193 and 7134. Virus titers are expressed as means ± standard error of eye swabs (n = 24) for 12 mice per group. (B) Reactivation of latent infection from trigeminal ganglia of mice following eye inoculation was determined by cocultivation at 30 days postinfection. Vero cell monolayers were used for KOS infection. Presence of reactivatable 7134 and CJ83193 viruses were assayed on U2CEP4R11 cell monolayers.