FIG. 4.
Reduction of challenge wild-type HSV-1 strain mP replication in the eye and trigeminal ganglion of mice immunized with CJ83193. Female BALB/c mice were immunized with either mock-infected cell lysate, KOS, d27, or CJ83193 at 2 × 106 PFU per mouse. Individual groups of mice (n = 12) were boosted with the same virus 2 weeks after primary immunization. At 4 weeks after primary immunization, mice in all groups were challenged following corneal scarification with HSV-1 strain mP. Eye swabs were taken on days 1, 3, 5, and 7 postchallenge, while mouse trigeminal ganglia (n = 8) were prepared on days 3, 5, and 7 postchallenge. Infectious viruses in individual eye swab materials (A) and trigeminal ganglia (B) were assessed by standard plaque assay on Vero cell monolayers. Viral titers are expressed as the mean ± standard error in individual eye swabs and trigeminal ganglia of mice per group.