Skip to main content
. 2004 Jun;78(11):5576–5583. doi: 10.1128/JVI.78.11.5576-5583.2004

FIG. 2.

FIG. 2.

Reactivity of cells expressing proteins from wild-type and mutant M viral RNA segments with anti-BM2 peptide serum in an immunofluorescence assay. 293T cells were transfected with four protein expression plasmids for the polymerase proteins and NP of B/Lee/40 and the wild-type or mutant BM2 PolI construct. Antigens were detected with rabbit anti-BM2 peptide serum as a primary antibody and FITC-conjugated anti-rabbit IgG as a secondary antibody. Wild-type, cells transfected with pPolBLeeM; #1, #2, #3, #4, 4Met−, and del, cells transfected with pPolBLeeBM2stop#1, pPolBLeeBM2stop#2, pPolBLeeBM2stop#3, pPolBLeeBM2stop#4, pPolBLeeBM2stop#1(4Met−), and pPolBLeeBM2del, respectively; mock, cells transfected with only the four protein expression plasmids for polymerase proteins and NP.