PCR gel electrophoresis results for detection of mycoplasma contamination with three different mycoplasma species in Vero cell line as positive control with mycoplasma species-specific primers. Lane 1 DNA size marker (100 bp DNA Ladder, Roche VIII), lane 2
M. hominis-specific primer (negative), lane 3
M. pirum-specific primer (negative), lane 4
M. pneumoniae-specific primer (negative), lane 5
A. laidlawii-specific primer (negative), lane 6
M. salivarium-specific primer (negative), lane 7
M. hyorhinis-specific primer (positive with Amplicone size 334 bp), lane 8
M. arginini-specific primer (positive with Amplicone size 326 bp), lane 9
M. fermentans-specific primer (positive with Amplicone size 324 bp), lane 10
M. orale-specific primer (negative), lane 11
M. genitalium-specific primer (negative), lane 12
U. urealyticum-specific primer (negative), lane 13 DNA-free water (negative control)