Table 1.

The details of alkaline comet assay analysis on HDF cells (early and late passages) and positive control (cells exposed by γ-radiation)

Parameters	Early passage of HDF	Late passage of HDF	γ-radiation
	No. of cells (100)	No. of cells (94)	No. of cells (70)
	Mean ± SD	Mean ± SD	Mean ± SD
Head area	1512.29 ± 725.854	805.63 ± 369.86	784.41 ± 140.26
Tail area	102.75 ± 99.158	2486.78 ± 676	8504 ± 2487.915
Head DNA	209.271 ± 101.533	86.887 ± 35.621	68.562 ± 28.461
Tail DNA	3.631 ± 4.137	120.777 ± 40.118	510.02 ± 90.75
Head DNA %	98.257 ± 1.8	41.436 ± 9.22	11.36 ± 3.62
Tail DNA %	1.743 ± 1.8	58.564 ± 9.22	88.64 ± 3.62
Tail length	3.44 ± 0.978	53.45 ± 8.928	116 ± 33.05
Comet length	48.46 ± 9.784	86.81 ± 11.125	148.66 ± 31.96
Tail moment	0.068 ± 0.0802	31.575 ± 8.638	102.24 ± 27.21

Head area: area of the comet head in pixels, Tail area: area of the comet tail in pixels, Head DNA: amount of DNA in the comet head, Tail DNA: amount of DNA in the comet tail, Tail Length: length of the comet tail measured from right border of head area to end of tail (in pixels), Comet length: length of the entire comet from left border of head area to end of tail (in pixels), Tail moment: tail DNA % × tail length [(percent of DNA in the tail) × (tail length)]

SD Standard deviation