Table 3.
Three-dimensional culture configurations enhancing stem cell properties
| Stem cell type | Bioreactor type | 3-D culture configuration | Effect on stem cells | Reference |
|---|---|---|---|---|
| MSCs | Spinner flask | Aggregates | Enhanced cell survival, VEGF secretion | Bhang et al. (2011) |
| MSCs | Spinner flask | Aggregates | Enhanced IL-24 secretion (anti-tumor factor) | Frith et al. (2010) |
| MSCs | Spinner flask | Aggregates | Enhanced osteogenesis | Frith et al. (2010) |
| MSCs | Rotating wall vessel | Aggregates | Enhanced adipogenesis | Frith et al. (2010) |
| MSCs | Spinner flask | Cytopore-2 microcarriers | Enhanced chondrogenesis compared to 2-D | Sart et al. (2013a) |
| MSCs | Spinner flask | Cytodex-3 microcarriers | Enhanced osteogenesis compared to 2-D | Goh et al. (2013) |
| mESCs | Static | Aggregates | Enhanced cell survival | Sart et al. (2013b) |
| mESCs | Spinner flask | Aggregates | Enhanced osteoblast differentiation | Alfred et al. (2010) |
| mESCs | Spinner flask | CultiSpher-S | Supported stem cell expansion | Storm et al. (2010) |
| hESCs | Static | Aggregates | Enhanced self-renewal | Singh et al. (2010) |
| hESCs | Spinner flask | Tosoh 10 microcarriers | Enhanced cardiomyogenesis compared to EBs | Lecina et al. (2010) |
| hESCs hiPSCs |
Spinner flask | DE-53 microcarriers | Enhanced neurogenesis compared to EBs | Bardy et al. (2013) |
MSCs mesenchymal stem cells, mESCs mouse embryonic stem cells, hESCs human embryonic stem cells, hiPSCs human induced pluripotent stem cells