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Cleavage pattern of wild-type and mutant S proteins in the presence or absence of dec-RVKR-cmk. Parallel cultures of LR-7 cells were infected with MHV-SA59H716D (H716D) or MHV-SA59R10 (R10), treated with the furin inhibitor, labeled, chased, and processed for immunoprecipitation and electrophoresis as described in the legend to Fig. 1, except the cells were starved at 6.5 h postinfection. m, mock-infected cells; p and c, pulse and chase samples, respectively.
