Table 1.
Specific metabolite utilization rates during the exponential growth (0–72 h) of the five insect cell lines grown in IBL-11 medium
| Metabolite | Metabolite specific utilization rate (10−15 mol cell−1 h−1) | |||||
|---|---|---|---|---|---|---|
| Glutamine-containing medium | Glutamine-free medium | |||||
| Se-1 | Se-El | Se-E4 | Se-E5 | Sf-21 | Sf-21 | |
| Ammonia | −39.67 ± 3.03 | −9.63 ± 0.32 | 1.89 ± 0.12 | −1.92 ± 0.07 | 22.95 ± 1.87 | 4.81 ± 0.36 |
| Glucose | 35.97 ± 0.97 | 97.24 ± 3.48 | 70.51 ± 1.49 | 81.86 ± 3.38 | 195.90 ± 7.53 | 59.62 ± 3.12 |
| Lactate | −3.75 ± 0.02 | −9.26 ± 0.05 | 4.48 ± 0.13 | −0.48 ± 0.03 | 9.28 ± 0.19 | 5.03 ± 0.38 |
| Alanine | −81.99 ± 0.28 | −15.90 ± 0.15 | −28.32 ± 1.00 | −51.19 ± 2.78 | −37.53 ± 1.53 | 9.84 ± 0.45 |
| Arginine | 2.03 ± 0.08 | 2.04 ± 0.06 | 3.30 ± 0.15 | 3.07 ± 0.02 | 8.80 ± 0.07 | 6.54 ± 0.51 |
| Asparagine | −0.54 ± 0.04 | 1.03 ± 0.01 | 1.11 ± 0.04 | 0.89 ± 0.03 | 2.48 ± 0.17 | 5.06 ± 0.23 |
| Aspartate | 3.10 ± 0.16 | 24.58 ± 1.30 | 10.05 ± 0.56 | 19.59 ± 1.61 | −0.42 ± 0.02 | 6.51 ± 0.54 |
| Glutamate | 23.84 ± 0.81 | 100.11 ± 6.35 | 59.30 ± 0.91 | 95.20 ± 5.41 | −9.79 ± 0.14 | 15.62 ± 1.36 |
| Glutamine | 47.96 ± 0.56 | 13.90 ± 0.37 | 19.14 ± 1.48 | 29.64 ± 2.19 | 44.37 ± 2.21 | n.d. |
| Histidine | −1.06 ± 0.01 | 0.42 ± 0.01 | 0.74 ± 0.02 | 0.42 ± 0.03 | 2.82 ± 0.23 | 4.47 ± 0.21 |
| Isoleucine | −1.78 ± 0.09 | 1.42 ± 0.03 | l.97 ± 0.12 | 1.39 ± 0.08 | 5.16 ± 0.22 | 7.34 ± 0.39 |
| Leucine | −6.55 ± 0.36 | 2.80 ± 0.08 | 3.36 ± 0.21 | 2.53 ± 0.16 | 5.48 ± 0.28 | 13.68 ± 1.25 |
| Lysine | −1.56 ± 0.08 | −0.39 ± 0.03 | 0.61 ± 0.03 | −1.22 ± 0.02 | −4.91 ± 0.37 | −4.31 ± 0.24 |
| Methionine | −2.12 ± 0.11 | 0.64 ± 0.04 | 1.84 ± 0.03 | 0.84 ± 0.02 | 1.94 ± 0.12 | 2.54 ± 0.18 |
| Phenylalanine | −1.59 ± 0.06 | 1.90 ± 0.15 | 4.53 ± 0.34 | 5.05 ± 0.33 | 2.71 ± 0.24 | 0.91 ± 0.03 |
| Proline | 1.31 ± 0.07 | −1.66 ± 0.09 | 2.42 ± 0.14 | 0.29 ± 0.02 | 12.12 ± 0.56 | 7.43 ± 0.56 |
| Serine | 2.93 ± 0.20 | 4.52 ± 0.37 | 7.43 ± 0.27 | 2.10 ± 0.01 | 18.9 ± 0.25 | 11.29 ± 0.88 |
| Threonine | 0.50 ± 0.04 | 1.62 ± 0.05 | 2.75 ± 0.11 | 2.78 ± 0.19 | 5.41 ± 0.01 | 5.30 ± 0.39 |
| Tryptophan | −1.15 ± 0.01 | 0.58 ± 0.05 | 1.87 ± 0.14 | 1.22 ± 0.04 | 1.55 ± 0.11 | 2.13 ± 0.12 |
| Tyrosine | 1.43 ± 0.01 | 0.18 ± 0.01 | −0.94 ± 0.04 | −0.46 ± 0.03 | 4.20 ± 0.21 | 5.16 ± 0.34 |
| Valine | −3.83 ± 0.15 | −0.06 ± 0.02 | 0.87 ± 0.05 | −0.17 ± 0.01 | 0.63 ± 0.05 | 1.53 ± 0.12 |
Positive values for the utilization rates indicate the consumption of metabolites, and negative values indicate the production of metabolites. Because glutamate and aspartate were consumed very rapidly in several of the five insect cell cultures (Fig. S1 g, h), their utilization rates were calculated during the initial growth phase (0–48 h). Each experiment on metabolite utilization was performed in duplicate and all data are presented as the mean ± SD