. 2013 Oct 5;66(5):729–740. doi: 10.1007/s10616-013-9639-2

Table 1.

The primer sequences for PCR and QRT-PCR

Gene	Primer (5′ → 3′)
β-Actin	Forward: GCGGCATCCACGAAACTAC
β-Actin	Reverse: TGATCTCCTTCTGCATCCTGTC
Oct4	Forward: GTGTTCAGCCAAAAGACCATCT
Oct4	Reverse: GGCCTGCATGAGGGTTTCT
Sox2	Forward: CCGAGTGGAAACTTTTGTCG
Sox2	Reverse: GGCAGCGTGTACTTATCCTTCT
c-Myc	Forward: GGACTTGTTGCGGAAACGAC
c-Myc	Reverse: ACTCAGCCAAGGTTGTGAGGT
Klf4	Forward: CCCACATGAAGCGACTTCCC
Klf4	Reverse: CAGGTCCAGGAGATCGTTGAA
Nanog	Forward: AAGGTCCCGGTCAAGAAACAG
Nanog	Reverse: CTTCTGCGTCACACCATTGC
Pax4	Forward: GGCACTGGAGAAAGAGTTCC
Pax4	Reverse: GGCACTGGAGAAAGAGTTCC
β-III-tubulin	Forward: CTTTTGGCCAGATCTTTAGACC
β-III-tubulin	Reverse: CTCGTTGTCAATGCAATAGGTC
Vimentin	Forward: GTCCAAGTTTGCCGACCTCT
Vimentin	Reverse: AGCGCATCCACTTCACAGG
NSE	Forward: CTGATGCTGGAGTTGGATGG
NSE	Reverse: CCATTGATCACGTTGAAGGC
miR302/367	Forward: GATCTCTAGATAATGTGGGTTTGCTCTTCTGTTT
miR302/367	Reverse: GATCGGATCCTAAGATGGGCGAGGAGGTTAT

PCR polymerase chain reaction