Figure 2. Leukocyte migration in experimental AAA.
A mixed population of spleen and bone marrow cells from aneurysmal mice were labeled with CFSE, and intravenously transferred into aneurysmal recipient mice pretreated with MKEY 30 min before cell transfer. Recipient mice were sacrificed 2 hours after cell transfer. Donor cells in the spleen, peripheral lymph nodes (PLN), bone marrow and aneurysmal aortae of recipient mice were evaluated using either flow cytometric or tissue immunofluorescence analysis. Migration of donor cells in MKEY-treated group was expressed as the percentage of that in vehicle-treated group, in which migration was set up at 100. Nonparametric Mann-Whitney test, *P<0.05 and **P<0.01 compared to vehicle-treated group. n=4 mice in each group.