Skip to main content
. Author manuscript; available in PMC: 2014 Sep 9.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Jan 3;33(4):718–726. doi: 10.1161/ATVBAHA.112.300329

Figure 4. Influence of MKEY treatment on AAA pathology.

Figure 4

Aortic sections from mice 2 wk after PPE infusion were stained with Elastin Masson stain for elastin fibers or immunostained with an antibody against SMC α-actin for SMCs, MAC2 for macrophages or CD31 for blood vessels. There were 8 mice in the vehicle group, 5 mice in 10 mg/kg MKEY treatment groups and 7 mice in 20 mg/kg MKEY treatment group.

(A): Representative aortic histology images for elastin, SMCs (SMC alpha actin), macrophages (MAC2) and blood vessels (CD31) from PPE-infused mice treated with vehicle or MKEY. Lum: lumen; Med: media; Adv: adventitia.

(B, C): Medial elastin fragmentation (C) and SMC destruction (D) were scored as mild (I) to severe (IV) using a histology grading system. Data are mean and SD of the scores in individual groups.

(D, E): MAC2+ macrophages and CD31+ blood vessels in media and adventitia were counted on each ACS, and data are given as mean and SD for macrophages or blood vessels per ACS. In all experiments, Nonparametric Mann-Whitney test, *P<0.05 and **P<0.01 between two groups.