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. 2014 Aug 24;15(1):709. doi: 10.1186/1471-2164-15-709

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© Hales et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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qPCR analysis of miR-200 family and in situ hybridization of miR200a. Expression of all three members of the chicken miR-200 family is significantly upregulated in ovarian tumors compared to normal ovaries. Expression of all three members is significantly decreased in ovarian tumors from flax-fed hens compared to tumors from control-fed hens. Expression of miR-200a is confined to the tumor cells of the control-fed ovary (A) and flax-fed ovary (B). Little if any expression is observed in normal ovary tissue (C). Magnification 20x on left, 100x on right.