Table 3.
Results from MS searching with the original Ensembl protein database and the reduced database
Sample | RNA-Seq reads | Mass spectra | Original database | Reduced database | |||
---|---|---|---|---|---|---|---|
# entries | Peptide IDs* | # entries | Peptide IDs* | % increase | |||
Jurkat human cells | 80 M | 500 K | 104,310 | 73,123 | 82,101 | 73,436 | 0.4 |
B6 mouse islets | 94 M | 250 K | 52,165 | 30,212 | 18,052 | 30,220 | 0.3 |
CAST mouse islets | 126 M | 250 K | 52,165 | 28,902 | 16,940 | 28,823 | 0.2 |
*peptide passing a 1% FDR.