Figure 5.

Expression levels of selected known miRNAs in leaf, flower and fruit. Total RNAs from mixed leaf, mixed flower and fruit in 170DAF were extracted for RNA gel blot analyses; reverse complement probes were used to detect the indicated miRNAs. U6 RNA or 5.8 s rRNA were used as loading controls. Two different genotypes of orange were used. Orange genotype 1 is a common sweet orange while genotype 2 is a red flesh orange. (a) miR482d-5p.2 was specifically expressed in leaf. (b) miR3954a and miR167b.2 were expressed at higher levels in flower than the other tissues; miR5179 was specifically expressed in flower (c) miR3951 and miR164 were expressed at higher levels in fruit than the other tissues; miR477a-3p was specifically expressed in fruit. L1, leaf of orange genotype 1; L2, leaf of orange genotype 2; F1, flower of orange genotype 1; F2, flower of orange genotype 2; Fr1, fruit of orange genotype 1; Fr2, fruit of orange genotype 2.