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. 2014 Aug 20;15(1):695. doi: 10.1186/1471-2164-15-695

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© Liu et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Csi-miR164 targeted Cs5g10870 was verified in vivo. (a) Expression levels of mi164 and Cs5g10870 were detected during the fruit development by qRT-PCR. U4 and ACTIN were used as loading controls in qRT-PCR Data from the qRT-PCR experiments are represented as the mean plus SD of n = 3 biological replicates. (b) Four overexpression vectors were constructed for transient expression system in tobacco. Vector ox-miR164 overexpress csi-miR164, ox-miRCon overexpress a control miRNA, ox-10TS + GFP overexpress a GFP gene carrying target site of miR164 in Cs5g10870, ox-10MTS + GFP overexpress a GFP gene carrying a modified target site. (c) Vectors used in tobacco co-expression assays in each lane were shown in the table. (d) Co-infiltrated leaves and control leaves were photographed at the 3rd day after infiltration under bright light. (e) Same leaves were photographed at the 3rd day after infiltration under UV light (wavelength = 365 nm).