Table 4.
Primary and secondary antibodies used for double labeling
| Antigen | Antigen retrieval | Species | Final dilution | Source primary antibody | Secondary antibody | Source secondary antibody | Conjugate | Source conjugate |
|---|---|---|---|---|---|---|---|---|
| Iba-1 | Tris-EDTA | Goat | 1:300 | Abcam, ab5076 | Alexa Fluor 488 coupled donkey anti goat IgG | Molecular Probes, A11055 | ||
| GFAP | Citrate | Rabbit | 1:4000 | DAKO, Z0334 | Alexa Fluor 594 coupled donkey anti rabbit IgG | Molecular Probes, A21207 | ||
| CCL2 | Citrate | Mouse | 1:200 | R&D Systems, MAB2791 | Biotinylated goat anti mouse IgG | Jackson, 115-065-146 | Alexa Fluor 488 coupled streptavidin | Molecular Probes, S11223 |
| CCR2 | Tris-EDTA | Rabbit | 1:1000 | Abcam, ab32144 | Alexa Fluor 594 coupled donkey anti rabbit IgG | Molecular Probes, A21207 | ||
| BM28 | Tris-EDTA | Mouse | 1:600 | BD Transduction Laboratories, 610700 | Biotinylated donkey anti mouse IgG | Jackson, 715-065-151 | Alexa Fluor 488 coupled streptavidin | Molecular Probes, S11223 |