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. 2014 Aug 30;14:231. doi: 10.1186/s12870-014-0231-5

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© Garcia-Molina et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Subcellular localization of GFP-tagged SPL7 protein derivatives transiently expressed in tobacco leaf-epidermal cells. (a) Graphic depiction of SPL7 protein derived polypeptides used in this work. The conserved domains (SBP; IRPGC; TMD) are indicated with grey boxes. The position of the amino- and carboxy-terminal amino acid residues relative to the full size SPL7 protein is provided. (b) Confocal microscopy reveales co-localization of a translational fusion between the predicted SPL7 transmembrane domain and GFP (GFP::TMD) with the plasma membrane marked with the styryl dye FM4-64 (c) Expression of the entire SPL7 coding sequence fused in frame to GFP either at the amino- or carboxi-terminal ends (GFP::SPL7 and SPL7::GFP) results in a dual localization within or around the nucleus, respectively. (d) The carboxi-terminal GFP-tagged SPL7 (SPL7::GFP) co-localizes with the endoplasmic reticulum marked through co-infiltration with an mCherry-tagged ER marker (ER-rk). In all cases, representative images of the GFP, FM4-64, chlorophyll and mCherry signals are shown together with the corresponding bright field and merged images. Scale bars, 10 μm in (b-d).