Figure 6.
An SPL7-SBP transgene results in constitutive detectable levels of nuclear localized protein in stably transformed Arabidopsis. Seven-day-old spl7-2 mutant seedlings expressing the GFP-tagged N-terminal part of SPL7, including the SBP-domain but excluding the presumed dimerization domain, were grown on ½ MS supplemented with BCS 50 μM, CuSO4 1 μM or 10 μM. Roots (a) and leaves (b) were examined using confocal microscopy. The insets in the upper row of panel (a) show a fluorescent nucleus at a higher magnification. GFP and chlorophyll fluorescence is provided together with bright field and merged images. Scale bars, 100 μm (a) and 10 μm (b).