FIG. 2.
Replication kinetics of mutant HIV determined by real-time PCR. Equal amounts of HIVNL4-3 (solid circles), NL4-3IN:M154I (open inverted triangles), and NL4-3IN:T66I-M154I (solid squares) were inoculated onto H9 cells in triplicate reactions. Next, 106 cells were lysed and real-time PCR was performed at each time point. (A) Infected-cell equivalents of minus-strand strong-stop DNA (AA55-M667) with standard curve (inset); (B) completely synthesized HIV cDNA (M661-M667) with standard curve (inset); (C) two-LTR-circle DNA (MH535-MH536) with standard curve (inset); (D) mean ratio of two-LTR-circle DNA to cDNA. Each point is the mean of triplicate infections, and error bars are ±1 standard deviation. The number of replicates for each standard curve is indicated in each insert.