FIG. 5.

Enzymatic activities of recombinant IN proteins. Increasing amounts of recombinant IN were incubated in the presence of oligonucleotide substrates for 1 h at 37°C in triplicate reactions. Products were separated by denaturing PAGE. (A) dBY-1 dumbbell disintegration substrate is comprised of viral DNA and target DNA components which, upon addition of IN, are resolved into their respective parts; (B and C) representative gels from disintegration assays are shown for reference IN (B) and IN containing T66I-M154I (C); (D) V1-V2, the 21-mer corresponding to the viral LTR, undergoes 3′-end processing and strand transfer in the presence of IN; (E) reference IN; (F) IN containing T66I-M154I. Numbers at the top of each lane indicate nanomolar enzyme concentrations. S, substrate control; *, substrate; →, disintegration product; −2→, 3′-end processing product; S.T.P., strand transfer products.