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Activities of recombinant IN proteins at 100 nM. A 100 nM concentration of each recombinant IN was incubated in the presence of: dBY-1 (A) or V1-V2 (B) substrates for 1 h at 37°C in triplicate reactions. Products were separated by denaturing PAGE. Lanes: 1, INC280S; 2, INT66I-M154I; 3, INM154I; 4, INS153Y; 5, INT66I; 6, reference IN. S, substrate control; *, substrate; →, disintegration product; −2→, 3′-end-processing product; S.T.P., strand transfer products.
